CT HB06278

Manufacturers and contract testing facilities are prohibited from using animal test methods when validated alternative methods are available, effective October 1, 2025

Medical research for disease diagnosis, treatment, prevention, and FDA-regulated drug/device development is exempt from the animal testing restrictions

Retail diesel fuel sold April through November must contain minimum biodiesel percentages: 5% by July 2025, 10% by July 2027, and 20% by July 2029

State fleet diesel vehicles, vessels, and construction equipment face accelerated biodiesel requirements: 5% by July 2025 and 20% by July 2027 during April-November months

Alternative test methods include computational toxicology, high-throughput screening, in vitro studies, and tiered testing approaches validated by organizations like the Interagency Coordinating Committee on the Validation of Alternative Methods