NC S516

Establishes statewide definitions of "biological sex," "male," "female," "man," "woman," "boy," "girl," "mother," and "father" in North Carolina General Statutes based on reproductive anatomy present at birth, excluding psychological or subjective gender identity

Requires single and multiple occupancy restrooms, changing facilities, and sleeping quarters in covered facilities (prisons, jails, juvenile detention centers, domestic violence centers, rape crisis centers, and public schools receiving state funds) to be used by only one biological sex at a time

Creates a private cause of action allowing individuals to sue covered facilities that permit or fail to prevent persons of the opposite biological sex from using sex-designated restrooms, changing facilities, or sleeping quarters, with remedies including actual damages, punitive damages, and attorneys' fees

Eliminates the option to change sex designation on birth certificates due to sex reassignment surgery and requires driver's licenses to display sex as reflected on the applicant's birth certificate

Takes effect October 1, 2025, with a three-year statute of limitations for civil claims (extended for minors until three years after reaching age of majority)