WI AB100

Requires public schools, charter schools, and private schools in parental choice programs to designate all interscholastic, intramural, and club athletic teams as male, female, or coed based on biological sex as stated on original birth certificates

Prohibits students of male biological sex from participating on athletic teams or sports designated for females

Creates a private right of action allowing female students to sue educational institutions for injunctive relief and damages if denied athletic opportunities due to violations, or if subjected to retaliation for reporting violations

Mandates that all school locker rooms be designated for exclusive use by one biological sex, with exceptions for custodial staff, medical emergencies, maintenance, and safety drills

Requires schools to provide reasonable accommodations (such as single-occupancy facilities) to students who submit written requests to avoid using locker rooms designated for their biological sex, with such requests protected as confidential pupil records